Imidazole CAS#288-32-4

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Chemical Name:Imidazole

CAS No.:288-32-4

Molecular Formula:C3H4N2

Molecular weight:68.08

Sample: Available

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Product Details

Products Description of Imidazole CAS#288-32-4

Imidazole, with the molecular formula C3H4N2, is an organic compound and a type of diazoles. It is a five-membered aromatic heterocyclic compound containing two meta-nitrogen atoms in the molecular structure. The unshared electron pair of the 1-position nitrogen atom in the imidazole ring participates in cyclic conjugation, and the electron density of the nitrogen atom is reduced, making it easy for the hydrogen on this nitrogen atom to leave in the form of hydrogen ions.

Imidazole is both acidic and alkaline, and can form salts with strong bases. The chemical properties of imidazole can be summarized as a combination of pyridine and pyrrole. These two structural units are precisely histidine in the enzyme as an acyl transfer reagent in the hydrolysis of lipids. Plays an important role in catalysis. Derivatives of imidazole exist in biological organisms and are more important than imidazole itself in scientific research and industrial production, such as DNA, hemoglobin, etc.



Imidazole Chemical Properties

Melting point 

88-91 °C(lit.)

Boiling point 

256 °C(lit.)

density 

1.01 g/mL at 20 °C

vapor pressure 

<1 mm Hg ( 20 °C)

refractive index 

1.4801

Fp 

293 °F

storage temp. 

Store below +30°C.

solubility 

H2O: 0.1 M at 20 °C, clear, colorless

pka

6.953(at 25℃)

form 

crystalline

color 

white

Specific Gravity

1.03

Odor

Amine like

PH Range

9.5 - 11

PH

9.5-11.0 (25℃, 50mg/mL in H2O)

Water Solubility 

633 g/L (20 ºC)

Sensitive 

Hygroscopic

λmax

λ: 260 nm Amax: 0.10
λ: 280 nm Amax: 0.10

Merck 

14,4912

BRN 

103853

Dielectric constant

23.0(Ambient)

Stability:

Stable. Incompatible with acids, strong oxidizing agents. Protect from moisture.

InChIKey

RAXXELZNTBOGNW-UHFFFAOYSA-N

LogP

-0.02 at 25℃

CAS DataBase Reference

288-32-4(CAS DataBase Reference)

NIST Chemistry Reference

1H-Imidazole(288-32-4)

EPA Substance Registry System

Imidazole (288-32-4)

 Safety Information

Hazard Codes 

C,Xi,T

Risk Statements 

36/38-63-34-22-20/21/22-61

Safety Statements 

26-36/37/39-45-22-36-27-53

RIDADR 

UN 2923 8/PG 3

WGK Germany 

1

RTECS 

NI3325000

Autoignition Temperature

480 °C

TSCA 

Yes

HazardClass 

8

PackingGroup 

III

HS Code 

29332990

Hazardous Substances Data

288-32-4(Hazardous Substances Data)

Toxicity

LD50 in mice (mg/kg): 610 i.p.; 1880 orally (Nishie)


 Product Application of Imidazole CAS#288-32-4

Imidazole is slightly more basic than pyrazole and can generate stable salts such as picrates and nitrates. One use of imidazole is in the purification of His-tagged proteins in metal chelate affinity chromatography (IMAC).

The tagged protein binds to the nickel ion medium in the bead holes on the surface of the chromatography column. Excess imidazole passes through the chromatography column, eluting the tagged protein coordinated with nickel to obtain high-purity target protein.


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