Imidazole CAS#288-32-4
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Chemical Name:Imidazole
CAS No.:288-32-4
Molecular Formula:C3H4N2
Molecular weight:68.08
Sample: Available
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Products Description of Imidazole CAS#288-32-4
Imidazole, with the molecular formula C3H4N2, is an organic compound and a type of diazoles. It is a five-membered aromatic heterocyclic compound containing two meta-nitrogen atoms in the molecular structure. The unshared electron pair of the 1-position nitrogen atom in the imidazole ring participates in cyclic conjugation, and the electron density of the nitrogen atom is reduced, making it easy for the hydrogen on this nitrogen atom to leave in the form of hydrogen ions.
Imidazole is both acidic and alkaline, and can form salts with strong bases. The chemical properties of imidazole can be summarized as a combination of pyridine and pyrrole. These two structural units are precisely histidine in the enzyme as an acyl transfer reagent in the hydrolysis of lipids. Plays an important role in catalysis. Derivatives of imidazole exist in biological organisms and are more important than imidazole itself in scientific research and industrial production, such as DNA, hemoglobin, etc.
Imidazole Chemical Properties
Melting point | 88-91 °C(lit.) |
Boiling point | 256 °C(lit.) |
density | 1.01 g/mL at 20 °C |
vapor pressure | <1 mm Hg ( 20 °C) |
refractive index | 1.4801 |
Fp | 293 °F |
storage temp. | Store below +30°C. |
solubility | H2O: 0.1 M at 20 °C, clear, colorless |
pka | 6.953(at 25℃) |
form | |
color | white |
Specific Gravity | 1.03 |
Odor | Amine like |
PH Range | 9.5 - 11 |
PH | 9.5-11.0 (25℃, 50mg/mL in H2O) |
Water Solubility | 633 g/L (20 ºC) |
Sensitive | Hygroscopic |
λmax | λ: 260 nm Amax: 0.10 |
Merck | 14,4912 |
BRN | 103853 |
Dielectric constant | 23.0(Ambient) |
Stability: | Stable. Incompatible with acids, strong oxidizing agents. Protect from moisture. |
InChIKey | RAXXELZNTBOGNW-UHFFFAOYSA-N |
LogP | -0.02 at 25℃ |
CAS DataBase Reference | 288-32-4(CAS DataBase Reference) |
NIST Chemistry Reference | 1H-Imidazole(288-32-4) |
EPA Substance Registry System | Imidazole (288-32-4) |
Safety Information
Hazard Codes | C,Xi,T |
Risk Statements | 36/38-63-34-22-20/21/22-61 |
Safety Statements | 26-36/37/39-45-22-36-27-53 |
RIDADR | UN 2923 8/PG 3 |
WGK Germany | 1 |
RTECS | NI3325000 |
Autoignition Temperature | 480 °C |
TSCA | Yes |
HazardClass | 8 |
PackingGroup | III |
HS Code | 29332990 |
Hazardous Substances Data | 288-32-4(Hazardous Substances Data) |
Toxicity | LD50 in mice (mg/kg): 610 i.p.; 1880 orally (Nishie) |
Product Application of Imidazole CAS#288-32-4
Imidazole is slightly more basic than pyrazole and can generate stable salts such as picrates and nitrates. One use of imidazole is in the purification of His-tagged proteins in metal chelate affinity chromatography (IMAC).
The tagged protein binds to the nickel ion medium in the bead holes on the surface of the chromatography column. Excess imidazole passes through the chromatography column, eluting the tagged protein coordinated with nickel to obtain high-purity target protein.
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