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Crystal Violet CAS#548-62-9

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Chemical Name：Crystal Violet

CAS No.548-62-9

Molecular Formula：C25H30ClN3

Molecular weight:407.99

Sample: Available

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Product Details

Products Description of Crystal Violet CAS#548-62-9

Gentian violet, also known as crystal violet and basic violet 3, is a commonly used artificial basic dye along with methyl green, neutral red, methylene blue, safranin, hematoxylin, malachite green, methyl violet and basic fuchsin. The dye index number is CIBasicViolet3(42555). It belongs to the triaminotriphenylmethane compound. It has the appearance of dark green powder or flaky crystals with bronze luster. The commercial product is a mixture of tetra-, penta- and hexamethyl basic fuchsin hydrochloride, in which the content of hexamethyl derivative is not less than 96%. Gentian violet is a mixed basic dye, mainly a mixture of crystal violet and methyl violet. When necessary, gentian violet can be used interchangeably with crystal violet. The main component of gentian violet used in medicine is methyl violet, which can replace gentian violet and crystal violet when needed. Gentian violet and acetic acid magenta are used for the color development reaction of cell chromosomes. They are often used to color chromatin or chromosomes when observing plant cell mitosis. The dilute solution of gentian violet is commonly known as gentian violet. Although the solution is slightly acidic when used for dyeing, it is an alkaline cationic dye because it has basic groups.

Soluble in water, ethanol and chloroform, insoluble in ether. Aqueous and ethanol solutions are dark purple, and turn orange in concentrated sulfuric acid. Dark green glittering powder or particles. Soluble in cold and hot water, purple, very soluble in ethanol. Reddish yellow in concentrated sulfuric acid, dark green yellow after dilution, and then turns blue and purple. The light fastness of dyeing cotton fiber is 1-2, and the light fastness of dyeing acrylic fiber is 2-3.

Crystal Violet Chemical Properties

Melting point	205 °C (dec.) (lit.)
Boiling point	560.86°C (rough estimate)
density	1.19 g/cm3 at 20 °C
vapor pressure	0Pa at 25℃
refractive index	1.6010 (estimate)
Fp	40 °C
storage temp.	room temp
solubility	water: soluble50g/L at 27°C
pka	9.4(at 25℃)
Colour Index	42555
form	Solid
color	S. No.: 785
PH	2.5-3.5 (10g/l, H2O, 20℃)
PH Range	0.8(yellow)-2.6(blue/violet)
Odor	Slight characteristic odor
Water Solubility	16 g/L (25 ºC)
λmax	590nm
Merck	14,4395
BRN	4077708
Stability:	Stable. Incompatible with strong oxidizing agents, strong acids. Light-sensitive. Combustible.
Biological Applications	Detecting microorganisms; treating atopic dermatitis,dermatological diseases,28,skin wounds,lesions,hemorrhoids,1,multiple myeloma,Non-Hodgkin’s lymphoma,breast cancer,neurodegenerative diseases,onychomycosis; wound dressing
Major Application	Photoresists, lithographic printing plate, printed circuit board, inks, hair dyes, shampoo, drug screening method, bone cement preparation method, microorganisms, hemorrhoids, antifungal, antibacterial, antimalarial agent, dental application
InChIKey	ZXJXZNDDNMQXFV-UHFFFAOYSA-M
LogP	1.172 at 25℃
CAS DataBase Reference	548-62-9(CAS DataBase Reference)
EPA Substance Registry System	Crystal violet (548-62-9)

Safety Information

Hazard Codes	Xn,N,T,C
Risk Statements	52/53-50/53-45-41-22-40-34-10-36/37/38-23/25-51/53-68
Safety Statements	61-60-53-45-46-36/37/39-26-39-36/37-16-22
RIDADR	UN 3077 9/PG 3
WGK Germany	3
RTECS	BO9000000
TSCA	Yes
HazardClass	9
PackingGroup	III
HS Code	32041300
Hazardous Substances Data	548-62-9(Hazardous Substances Data)
Toxicity	LD50 orally in mice, rats: 1.2, 1.0 g/kg (Hodge)

Product Application of Crystal Violet CAS#548-62-9

Used as an acid-base indicator, the pH color change range is 0.1 (green) to 2.0 (blue). In analytical chemistry, it is used as a non-aqueous solution titration indicator, a biological stain, a colorimetric assay developer, and is also used to test ions such as thallium, zinc, antimony, titanium, cadmium, tungsten, gold, and mercury. Crystal violet is also an important dye in bacterial Gram staining. It can be used as a dye for silk, paper, acrylic fiber, etc. The solubility of its chloride in water is 1.68%, and the solubility in 95% ethanol is 13.87%; the solubility of its iodide in the two solvents is 0.035% and 1.78%, respectively. The mixed reagent of crystal violet chloride and iodide is called gentian violet. Commonly used dyes for bacterial staining specimens include alkaline fuchsin, crystal violet, methylene blue, etc.

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